Whole Genome Sequencing to study the phylogenetic structure of serotype a Haemophilus influenzae recovered from patients in Canada

This study examined the phylogenetic structure of serotype a Haemophilus influenzae (Hia) isolates recovered from patients in Canada. Hia isolates from 490 separate patients and an ATCC strain were analyzed by multi-locus sequence typing (MLST) with 18 different sequence types (STs) identified. Most (85.7%) Hia patient isolates were typed as ST-23 and another 12.7% belonged to 14 different STs with six, five, or four MLST gene loci related to ST-23 (ST-23 complex). Core genome single nucleotide variation phylogeny (SNVPhyl) on whole genome sequence (WGS) data of 121 Hia patient isolates representing all identified STs and the ATCC strain revealed two phylogenetic populations with all the ST-23 complex isolates within one population. The other phylogenetic population contained only the ATCC stain and three patient isolates. Concatenated hitABC sequences retrieved from WGS data and analyzed by MEGA alignment confirmed the phylogeny obtained by SNVPhyl. sodC genes were found only in isolates in the minor phylogenetic population. The two phylogenetic populations of the Canadian Hia isolates are similar to the two clonal divisions described for serotype b H. influenzae. Combining MLST, core SNVPhyl, and hitABC gene sequence alignment showed that most (99.4%) Canadian Hia patient isolates belonged in one major phylogenetic population.
Auteurs (Zotero)
Tsang, Raymond S. W.; Shuel, Michelle; Ahmad, Tauqeer; Hayden, Kristy; Knox, Natalie C.; Van Domselaar, Gary; Hoang, Linda; Tyrrell, Gregory; Minion, Jessica; Vancaeseele, Paul; Kus, Julianne V.; Ulanova, Marina; Lefebvre, Brigitte; Haldane, David Jm; Garceau, Richard; German, Greg; Zahariadis, George; Hanley, Brendan; Kandola, Kami; Patterson, Michael
Date de publication (Zotero)
octobre, 2019